Membrane phospholipid synthesis and phenotypic correlation of an Escherichia coli pss mutant

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Membrane phospholipid synthesis and phenotypic correlation of an Escherichia coli pss mutant.

A pair of putatively isogenic pss(Ts) and pss+ (phosphatidylserine synthetase structural gene) strains was constructed and analyzed, together with the revertants, for the physiological consequences of cessation of the optimal synthesis of phosphatidylethanolamine (PE). Their in vivo and in vitro abilities to synthetize PE and the growth rates at different temperatures were determined. The rate ...

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Membrane phospholipid synthesis in Escherichia coli. Purification, reconstitution, and characterization of sn-glycerol-3-phosphate acyltransferase.

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Genetic manipulation of membrane phospholipid composition in Escherichia coli: pgsA mutants defective in phosphatidylglycerol synthesis.

Unique mutants of Escherichia coli K-12, defective in phosphatidylglycerol synthesis, have been isolated from a temperature-sensitive strain incubated at its nonpermissive temperature. The parent strain had excess phosphatidylglycerol by harboring both the pss-1 allele [coding for a temperature-sensitive phosphatidylserine synthase (EC 2.7.8.8)] and the cls- allele (responsible for a defective ...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1977

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.132.2.434-443.1977